Patent Number: 6,309,602

Title: Stacked, reconfigurable system for electrophoretic transport of charged materials

Abstract: Methods, apparatus, and applications for use of a stacked, reconfigurable system for electrophoretic transport are provided. In one embodiment, a system having a first chamber including at least a bottom support and an intermediate support, and a second chamber, said second chamber including a bottom support and a top member, the first and second chambers being coupled through a via. Electrophoretic, and optional electro-osmotic and thermal, transport is effected. In another aspect of this invention, three or more chambers are coupled by an electrophoretic buss. The electrophoretic buss includes driving electrodes and is adapted to receive fluid containing materials for transport. The chambers are coupled to the electrophoretic buss and serve as a tap from the buss for delivery of charged materials. In one embodiment, certain functions are performed in different chambers. For example, the first chamber may receive the sample and perform sample processing functions, the second chamber may perform amplification procedures, yet a third chamber may perform hybridization or other assays, and yet another chamber may perform immunoassays. By separating various functions to different chambers, speed and sensitivity may be improved. In yet another aspect of this invention, analysis from a earlier stage may be utilized in a subsequent stage to reconfigure the system for optimum use. In one application, analysis at a first level is utilized to determine an action at a second level, such as the synthesis of a compound. The synthesized compound in response to a biohazard may comprise vaccine or antidote.

Inventors: Ackley; Donald E. (Cardiff, CA), Sheldon; Edward L. (San Diego, CA), Krihak; Michael K. (San Diego, CA)

Assignee: Nanogen, Inc.

International Classification: B01L 3/00 (20060101); B01J 19/00 (20060101); C07B 61/00 (20060101); C07H 21/00 (20060101); C07K 1/00 (20060101); C07K 1/04 (20060101); C12Q 1/68 (20060101); F04B 19/00 (20060101); G01N 27/447 (20060101); G11C 13/02 (20060101); H01L 21/70 (20060101); H01L 21/02 (20060101); H01L 29/66 (20060101); H01L 21/336 (20060101); G11C 19/00 (20060101); H01L 21/98 (20060101); H01L 29/78 (20060101); G01N 015/00 (); G01N 001/00 (); B01J 008/00 ()

Expiration Date: 10/30/2018