Patent Number: 6,309,824

Title: Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes

Abstract: The present invention provides a method for detecting a target nucleic acid species including the steps of providing an array of probes affixed to a substrate and a plurality of labeled probes wherein each labeled probe is selected to have a first nucleic acid sequence which is complementary to a first portion of a target nucleic acid and wherein the nucleic acid sequence of at least one probe affixed to the substrate is complementary to a second portion of the nucleic acid sequence of the target, the second portion being adjacent to the first portion; applying a target nucleic acid to the array under suitable conditions for hybridization of probe sequences to complementary sequences; introducing a labeled probe to the array; hybridizing a probe affixed to the substrate to the target nucleic acid; hybridizing the labeled probe to the target nucleic acid; affixing the labeled probe to an adjacently hybridized probe in the array; and detecting the labeled probe affixed to the probe in the array.

Inventors: Drmanac; Radoje T. (Palo Alto, CA)

Assignee: Hyseq, Inc.

International Classification: C12Q 1/68 (20060101); C12Q 001/68 (); C07H 021/04 (); C12P 019/34 ()

Expiration Date: 10/30/2018