Patent Number: 6,309,833

Title: Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures

Abstract: A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.

Inventors: Edman; Carl F. (San Diego, CA), Nerenberg; Michael I. (La Jolla, CA), Westin; Lorelei P. (La Mesa, CA), Carrino; John J. (San Diego, CA)

Assignee: Nanogen/Becton Dickinson Partnership

International Classification: C12Q 1/68 (20060101); C12Q 001/68 (); C12P 019/34 ()

Expiration Date: 10/30/2018