Patent Number: 6,309,839

Title: Screening methods for compounds that inhibit or stimulate helicase enzyme activity

Abstract: The gp4l and Dda helicases were found to significantly enhance the dissociation rate of streptavidin from biotin-labeled oligonucleotides in an ATP dependent reaction, demonstrating that these enzymes are capable of imparting a significant force on a molecule blocking their path. The present invention describes an assay for studying enzymatic activity of a helicase using the rate of dissociation of streptavidin from biotinylated oligonucleotides.

Inventors: Raney; Kevin (Little Rock, AR), Morris; Patrick (Little Rock, AR), Dennis; Regina (Little Rock, AR)

Assignee: Board of Trustees of the University of Arkansas

International Classification: C12Q 1/533 (20060101); C12Q 1/68 (20060101); C12Q 001/68 (); G01N 033/53 (); C12P 019/34 (); C12N 009/00 (); C07H 021/02 ()

Expiration Date: 10/30/2018