Patent Number: 6,309,852

Title: Method and reagent for quantitative determination of 1,5-anhydroglucitol

Abstract: The present invention provides a simple method for the determination of aspecific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a samplecontaining glucose, and a reagent and a reagent kit useful in the method.In one embodiment, a method for the determination of 1,5-AG is providedwhich comprises contacting the sample with an enzyme system which convertsglucose into fructose-1,6-diphosphate and converts 1,5-AG into1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphatedehydrogenase in the presence of an oxidized coenzyme, and measuring theamount of the reduced coenzyme formed by the dehydrogenation reaction. Areagent and a reagent kit useful in this method are also provided.

Inventors: Tazoe; Sakae (Fuji, JP), Miike; Akira (Shizuoka, JP)


International Classification:

Expiration Date: 10/32013