Patent Number: 6,309,864

Title: Process for producing foreign proteins

Abstract: A production method of a heterologous protein, comprising culturing a heterologous protein-producing host prepared by gene manipulation in a medium containing a fatty acid or a salt thereof, and a surfactant and harvesting the heterologous protein from the culture. The production amount of the heterologous protein produced by a heterologous protein-producing host can be increased. Moreover, since decomposition of the heterologous protein by an enzyme derived from the host can be inhibited, enabling large scale production of a heterologous protein.

Inventors: Ohda; Toyoo (Hirakata, JP), Ohya; Tomoshi (Hirakata, JP), Kuwae; Shinobu (Hirakata, JP), Ohyama; Masao (Hirakata, JP), Kobayashi; Kaoru (Hirakata, JP), Uemura; Yahiro (Hirakata, JP)

Assignee: Yoshitomi Pharmaceutical Industries, Ltd.

International Classification: C12P 21/02 (20060101); C12N 1/16 (20060101); C12P 021/06 (); C07K 014/00 ()

Expiration Date: 10/30/2018