Patent Number: 6,309,883

Title: Methods and compositions for cellular and metabolic engineering

Abstract: The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to "evolve" individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.

Inventors: Minshull; Jeremy (San Francisco, CA), Stemmer; Willem P. C. (Los Gatos, CA)

Assignee: Maxygen, Inc.

International Classification: C07K 14/435 (20060101); C07K 14/545 (20060101); C07K 16/00 (20060101); C12N 9/86 (20060101); C12N 9/78 (20060101); C12N 15/52 (20060101); C12Q 1/68 (20060101); C12N 15/10 (20060101); C12N 9/38 (20060101); C12N 015/00 (); C12Q 001/68 (); C07H 021/02 (); C07H 021/04 ()

Expiration Date: 10/30/2018