Patent Number: 6,310,180

Title: Method for synthesis of proteins

Abstract: A method for peptide synthesis is disclosed that requires neither protecting groups nor activation of the C-.alpha. carboxyl groups. The method comprises ligating a first molecule to a second molecule by promoting the orthogonal coupling of the molecules to each other. In an aspect of this method, an acyl-type reaction occurs between the molecules. The method contemplates the joining of molecules of variant size to each other, as well as the coupling of multiple identical molecules. The invention also covers the ligation of unprotected peptide, proteins or nonpeptide segments to prepare therapeutic products and synthetic vaccines with linear, circularized, or branched backbone structures, as well as the site-specific modification of peptides or proteins by lipidation and pegylation.

Inventors: Tam; James P. (Nashville, TN)

Assignee: Vanderbilt University

International Classification: C07K 7/64 (20060101); C07K 7/00 (20060101); C07K 14/15 (20060101); C07K 1/107 (20060101); C07K 14/005 (20060101); C07K 14/16 (20060101); C07K 1/00 (20060101); C07K 1/08 (20060101); G01N 33/531 (20060101); A61K 38/00 (20060101); A61K 038/00 (); A61K 038/04 (); C07K 001/00 (); C07K 014/00 ()

Expiration Date: 10/30/2018