Patent Number: 6,310,271

Title: Polynucleotides encoding choline monooxygenase and plants transformed therewith

Abstract: A full length choline monooxygenase (CMO) cDNA was cloned from spinach and used to transform plants which do not naturally express CMO. A method is presented to improve stress tolerance of crops following engineering of CMO and BADH in plants that lack glycine betaine accumulation. Also provided are fragments useful as probes to isolate other CMO-type genes, and antisense sequences which inhibit the production of CMO. Reduction of glycine betaine as a consequence of antisense expression of CMO in species naturally accumulating glycine betaine, improves the transgenic plant's tolerance toward pathogens and pests and/or enhances its nutritional quality.

Inventors: Hanson; Andrew D. (Gainesville, FL), Rathinasabapathi; Bala (Gainesville, FL), Burnet; Michael (Les Hameaux, FR)

Assignee: University of Florida Research Foundation, Inc.

International Classification: C12N 15/82 (20060101); C12N 9/04 (20060101); C12N 015/29 (); C12N 005/04 (); C12N 015/82 (); A01H 005/00 (); A01H 005/10 ()

Expiration Date: 10/30/2018