Patent Number: 6,333,174

Title: Bacterial polypeptide expression employing tryptophan promoter-operator

Abstract: Methods for the controllable expression in high yields of heterologous proteins under the control of a modified tryptophan promoter-operator system are disclosed. The system employed involves sequential resort to tryptophan repression and derepression, as well as deletion of an attenuator function native to the tryptophan operon. Means are also disclosed for effecting deletions at any given point within a gene fragment and for the ready identification of transformant bacteria via triple ligation procedures.

Inventors: Kleid; Dennis G. (San Mateo, CA), Yansura; Daniel G. (San Francisco, CA), Heyneker; Herbert L. (Burlingame, CA), Miozzari; Giuseppe F. (San Carlos, CA)

Assignee: Genentech, Inc.

International Classification: C07K 14/435 (20060101); C07K 14/655 (20060101); C07K 14/61 (20060101); C12P 19/34 (20060101); C12P 19/00 (20060101); C12N 15/62 (20060101); C12N 15/71 (20060101); C12N 15/10 (20060101); C12N 015/63 (); C12N 015/71 ()

Expiration Date: 12/25/2018