Patent Number: 6,428,986

Title: Methods for DNA amplificating and sequencing

Abstract: The osmoprotectants proline, 2-methyl-4-carboxy-3,4,5,6-tetrahydropyrimidine ("THP(B)", and 2-methyl-4-carboxy-5-hydroxy-3,4,5,6,-tetrahydropyrimidine ("THP(A)") are capable of increasing the thermal stability of DNA polymerases at elevated temperatures. THP(B) is further effective in lowering the melting temperature of double-stranded DNA. Proline, THP(A) and THP(B) are thus useful in procedures involving melting of double-stranded DNA and/or polymerase-mediated DNA synthesis, such as in primer extension, in PCR (polymerase chain reaction) amplification and in DNA sequencing.

Inventors: Lapidot; Aviva (Rehovot, IL), Iakobashvili; Robert (Ashdod, IL), Malin; Gennady (Boston, MA)

Assignee: Yeda Research and Development Co., Ltd. at the Weizmann Institute of

International Classification: C12Q 1/68 (20060101); C12P 019/34 (); C12Q 001/68 ()

Expiration Date: 08/06/2019