Patent Number: 6,512,098

Title: Chemically modified proteins with a carbohydrate moiety

Abstract: The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine in a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also, a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins.

Inventors: Jones; J. Bryan (Lakefield, Ontario, CA), Davis; Benjamin G. (Durham, GB)

Assignee: Genencor International, Inc.

International Classification: C12N 9/54 (20060101); C12N 9/52 (20060101); C07K 001/00 (); C07K 014/00 (); C07K 017/00 (); C07H 021/02 (); C07H 021/04 ()

Expiration Date: 01/28/2020