Patent Number: 6,967,087

Title: Obligately symbiotic thermophile symbiobacterium toebii SC-1 producing thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase and a method for screening its relative bacteria

Abstract: The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebu SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

Inventors: Sung; Moon-Hee (Daejeon-si, KR), Lee; Seung-Goo (Daejeon-si, KR), Rhee; Sung-Keun (Daejeon-si, KR), Hong; Seung-Pyo (Daejeon-si, KR), Kang; Dae-Joong (Seoul-si, KR), Kim; Su-Mi (Daejeon-si, KR), Kim; Chul-Joong (Daejeon-si, KR), Bae; Jin-Woo (Daejeon, KR), Jeon; Che Ok (Ithaca, NY), Kim; Joong-Jae (Daegu, KR), Kim; Kwang (DaeJeon, KR), Song; Jae Jun (DaeJeon, KR)

Assignee: Korea Research Institute of Bioscience and Biotechnology

International Classification: C12N 9/88 (20060101); C12P 13/00 (20060101); C12P 13/22 (20060101); C12P 039/00 (); C12Q 001/06 (); C12Q 001/12 (); C12N 001/20 ()

Expiration Date: 1/22/02018