Patent Number: 7,045,319

Title: Molecular detection systems utilizing reiterative oligonucleotide synthesis

Abstract: The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.

Inventors: Hanna; Michelle M. (Phoenix, AZ)

Assignee: Ribomed Biotechnologies, Inc.

International Classification: C12P 19/34 (20060101); C12Q 1/68 (20060101)

Expiration Date: 5/16/02018