Patent Number: 7,045,353

Title: Directed differentiation of human embryonic cells

Abstract: Methods are described for mapping a pathway of differentiation of a population of embryonic cells which includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies which are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided.

Inventors: Benvenisty; Nissim (Jerusalem, IL)

Assignee: Yissum Research Development Company of The Hebrew University of Jerusalem

International Classification: C12N 5/02 (20060101); C12N 5/08 (20060101)

Expiration Date: 5/16/02018