Patent Number: 7,087,396

Title: Monoclonal antibody and method and kit for immunoassay of soluble human ST2

Abstract: A method for determining a soluble human ST2 in a sample conveniently at a high sensitivity and an assay kit are provided. By an immunological method comprising a step for bringing a sample into contact with an immobilized antibody formed by binding to an insoluble support a first anti-human ST2 antibody which binds specifically to a non-denatured human ST2, a step for labelling a first reaction product generated in the previous step by reacting said first reaction product with a second anti-human ST2 antibody which binds specifically to a non-denatured human ST2 by recognizing a site different from the site on ST2 where said first anti-human ST2 antibody binds and which is labelled with a label, and a step for determining the amount of the label on said first reaction product which has been labelled, a soluble human ST2 in a sample is determined. In addition, a recombinant ST2 is employed as a standard to prepare a calibration curve, based on which the ST2 in a sample is quantified.

Inventors: Tominaga; Shin-ichi (Yokohama-shi, Kanagawa, 224-0004, JP), Arai; Takao (Tokyo179-0074, JP), Kuroiwa; Kenji (Numazu, JP), Oshikawa; Katsuhisa (Kawachi, JP)

Assignee: Medical Biological Laboratories Co., Ltd.

International Classification: C07K 16/28 (20060101); C12N 5/20 (20060101); G01N 33/535 (20060101); G01N 33/543 (20060101); G01N 33/577 (20060101)

Expiration Date: 8/08/02018