Patent Number: 7,132,273

Title: Cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same

Abstract: The present invention provides novel cell wall anchor proteins derived from yeast, genes thereof and the genetic method for tethering polypeptide to the yeast cell wall using the same. Particularly, the present invention provides the novel GPI (glycosyl phosphatidyl inositol)-anchor protein genes, SFD1, GAS1, TIP1 and CWP1, and their proteins, PIR2 cell wall protein gene and its protein derived from Hansenula polymorpha, and the cell surface expression system using them which immobilize foreign enzymes or polypeptides on the cell wall of a microbial cell. In addition, the present invention provides the cell surface expression system using WSC1 gene and its protein derived from yeasts, including Hansenula polymorpha and Saccharomyces cerevisiae, and STA1 gene and its protein derived from Saccharomyces diastaticus. The cell surface expression system of the present invention expects an immobilization effect as biocatalysts by adhereing a desired protein to the cell surface, and provides a means of altering target protein characteristics such as binding affinity and stability by library screening.

Inventors: Choi; Eui-Sung (Daejon-si, KR), Sohn; Jung-Hoon (Daejon-si, KR), Kim; So-Young (Kimpo-si, KR)

Assignee: Korea Research Institute of Bioscience and Biotechnology

International Classification: C12N 1/20 (20060101); C07H 21/04 (20060101); C07K 1/00 (20060101); C12N 1/14 (20060101); C12N 15/00 (20060101); C12P 21/04 (20060101); C12Q 1/00 (20060101); C12Q 1/68 (20060101)

Expiration Date: 2019-11-07 0:00:00