Patent Number: 7,132,397

Title: Bone morphogenetic protein antagonist based on the mature protein

Abstract: The purpose is to provide a mature protein having an antagonistic activity against bone morphogenetic proteins. The mature protein having an antagonistic activity against bone morphogenetic proteins is obtained by converting at least one residue among methionine residues or tryptophane residues existing in the amino acid sequence of mature human MP52 (SEQ ID No 1) to a hydrophilic residue by chemical modification, or replacing said residues with a hydrophilic amino acid residue or a polar amino acid residue. The chemical modification for said methionine residue is performed by an oxidization reaction or an alkylation reaction. The chemical modification for said tryptophane residue is performed by an allylsulphenylation reaction. Or a mature protein having an antagonistic activity against bone morphogenetic proteins is obtained by converting at least one residue of tryptophane residues existing in the amino acid sequences of mature human BMP-2 (SEQ ID No 2), mature human BMP-4 (SEQ ID No 3), and mature human BMP-7 (SEQ ID No 4) to a hydrophilic residue by chemical modification, or replacing said residues with a hydrophilic amino acid residue or a polar amino acid residue.

Inventors: Katsuura; Mieko (Tokyo, JP), Kimura; Michio (Kanagawa, JP)

Assignee: Biopharm Gesellschaft zur biotechnologischen Entwicklung von Pharmaka mbH

International Classification: A61K 38/18 (20060101); C07K 14/51 (20060101)

Expiration Date: 2019-11-07 0:00:00