Patent Number: 7,132,527

Title: Promoter and plasmid system for genetic engineering

Abstract: This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Inventors: Payne; Mark S. (Wilmington, DE), Picataggio; Stephen K. (Landenbert, PA), Kuang-Hsu; Amy (Redwood City, CA), Nair; Ramesh V. (Cupertino, CA), Valle; Fernando (Burlingham, CA), Soucaille; Philippe (Deyme, FR), Trimbur; Donald E. (Landenberg, PA)

Assignee: E. I. du Pont de Nemours and Company

International Classification: C07H 21/04 (20060101); C12N 1/20 (20060101); C12N 15/00 (20060101); C12N 15/09 (20060101); C12N 15/63 (20060101); C12N 15/70 (20060101); C12N 15/74 (20060101)

Expiration Date: 2019-11-07 0:00:00