Patent Number: 7,202,063

Title: Processes for the production of rhamnolipids

Abstract: A process for producing rhamnolipids involving culturing Pseudomonas chlororaphis strain NRRL B-30761 in a first aqueous culture medium containing about 0.3% NH.sub.4H.sub.2PO.sub.4, about 0.2% K.sub.2HPO.sub.4, about 0.2% carbon source, about 0.5 mg/L FeSO.sub.4, and about 0.1% MgSO.sub.4 for about 24 about 48 hours at about 25.degree. about 30.degree. C. with orbital shaking, and then culturing Pseudomonas chlororaphis strain NRRL B-30761 in a static second aqueous culture medium containing per liter about 2% carbon source, about 0.7 g KH.sub.2PO.sub.4, about 0.9 g Na.sub.2HPO.sub.4, about 2 g NaNO.sub.3, about 0.4 g MgSO.sub.4.7H.sub.2O, and about 0.1 g CaCl.sub.2.2H.sub.2O for at least about 72 hours at about 20.degree. about 23.degree. C., wherein the first and second aqueous culture medium contains only one source of carbon.

Inventors: Gunther; Nereus W. (Newtown Square, PA), Solaiman; Daniel K. Y. (Dresher, PA), Fett; William F. (Blue Bell, PA)

Assignee: United States of America as represented by the Secretary of Agriculture

International Classification: C12P 7/64 (20060101); C12N 1/20 (20060101); C12P 17/02 (20060101); C12P 19/02 (20060101)

Expiration Date: 2019-04-10 0:00:00