Patent Number: 7,611,835

Title: Process for preparing multilayer enzyme coating on a fiber

Abstract: A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme "seeds" are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.

Inventors: Kim; Jungbae (Richland, WA), Kwak; Ja Hun (Richland, WA), Grate; Jay W. (West Richland, WA)

Assignee: Battelle Memorial Institute

International Classification: C12Q 1/00 (20060101); C12M 1/00 (20060101); C12M 1/34 (20060101); C12M 1/40 (20060101); C12N 11/02 (20060101); C12N 11/08 (20060101); C12N 11/14 (20060101); C12P 1/00 (20060101)

Expiration Date: 1/03/02017