Patent Number: 7,611,854

Title: Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor

Abstract: Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of .alpha.-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.

Inventors: Fowlkes; Dana M. (Chapel Hill, NC), Broach; Jim (Princeton, NJ), Manfredi; John (Ossining, NY), Klein; Christine (Ossining, NY), Murphy; Andrew J. (Montclair, NJ), Paul; Jeremy (South Nyack, NY), Trueheart; Joshua (South Nyack, NY)

Assignee: Cadus Technologies, Inc.

International Classification: C12N 1/19 (20060101); G01N 33/53 (20060101)

Expiration Date: 1/03/02017