Patent Number: 7,704,739

Title: Method of isolating and culturing mesenchymal stem cell derived from umbilical cord blood

Abstract: The present invention relates to a method of isolating and culturing mesenchymal stem cells using umbilical cord blood that is most ideal for cell therapy. The method comprises adding an anti-coagulant to umbilical cord blood having a volume of more than 45 ml per unit, which is obtained within 24 hours after parturition; diluting the resulting mixture of the anti-coagulant and umbilical cord blood with an .alpha.MEM (alpha-minimum essential medium), followed by centrifugation to harvest monocytes; and subjecting the obtained monocytes into suspension culture in the .alpha.MEM containing Stem Cell Factor, GM-CSF (granulocyte-macrophage colony-stimulating factor), G-CSF (granulocyte colony-stimulating factor), IL-3 (interleukin-3) and IL-6 (interleukin-6).

Inventors: Han; Hoon (Gyeonggi-do, KR), Kim; Sung-Whan (Seoul, KR)

Assignee: Han; Hoon

International Classification: C12N 5/00 (20060101)

Expiration Date: 4/27/12018