Patent Number: 7,709,188

Title: Multi-allelic detection of SARS-associated coronavirus

Abstract: The subject invention relates to a multiple-allelic RT-real-time polymerase chain reaction (PCR) assay for coronaviruses including the SARS virus. Multiple target sequences within the SARS-CoV, S, E, M and N genes are identified. The use of the four different targets enhances the likelihood that the fundamental genetic drift of the virus will not lead to a false negative result. Multiplex assays format for the assay are envisioned. Thus, the present invention allows for early diagnosis of a SARS infection. The assay would be useful in the context of monitoring treatment regimens, screening potential anti SARS agents, and similar applications requiring qualitative and quantitative determinations.

Inventors: Kostrikis; Leondios G. (Kapsalos Limassol, CY)

Assignee: Birch Biomedical Research LLC

International Classification: C12Q 1/00 (20060101); C12Q 1/68 (20060101); C12Q 1/70 (20060101)

Expiration Date: 5/04/12018