Patent Number: 7,711,491

Title: Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis

Abstract: A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.

Inventors: Vandersall; Jennifer A. (Livermore, CA), Gardner; Shea N. (San Leandro, CA), Clague; David S. (Livermore, CA)

Assignee: Lawrence Livermore National Security, LLC

International Classification: G01N 33/48 (20060101); C12N 15/11 (20060101); C12Q 1/68 (20060101)

Expiration Date: 5/04/12018