Patent Number: 7,713,739

Title: Microparticle-based transfection and activation of dendritic cells

Abstract: The present invention provides an effective method for the transfection of dendritic cells by non-viral methods. The present invention provides this benefit by incubating dendritic cells and a specified transfection agent. The transfection agent comprises a polynucleotide and microparticles, with the microparticles being comprised of biodegradable polymer and cationic detergent. The dendritic cells and transfection agent are incubated for a time sufficient to transfect the dendritic cells with the polynucleotide.

Inventors: Donnelly; John J. (Moraga, CA), Denis-Mize; Kimberly S. (Concord, CA), Ott; Gary S. (Oakland, CA)

Assignee: Novartis Vaccines and Diagnostics, Inc.

International Classification: C12N 15/64 (20060101); C12N 15/63 (20060101); C12N 5/10 (20060101)

Expiration Date: 5/11/12018