Patent Number: 7,732,585

Title: Altered recombinases for genome modification

Abstract: The present invention describes methods of identifying altered recombinases and compositions thereof, wherein at least one amino acid is different from a parent, wild-type recombinase and the altered recombinase has improved recombination efficiency towards wild-type and/or pseudo att site sequences relative to the parent, wild-type recombinase. The present invention also includes methods of modifying the genomes of cells using the altered recombinases, including methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell.

Inventors: Calos; Michele Pamela (Burlingame, CA)

Assignee: The Board of Trustees of the Leland Stanford Junior University

International Classification: C07H 21/04 (20060101); C12N 15/52 (20060101); C12N 15/63 (20060101)

Expiration Date: 2022-06-08 0:00:00