Patent Number: 7,733,483

Title: Method for ascertaining the orientation of molecules in biological specimens

Abstract: A method of ascertaining the orientation of molecules in a biological specimen by total internal reflection includes focusing illuminating light through an objective in different positions in a plane of a pupil of the objective so as to generate a plurality of respective differently oriented evanescent fields in the specimen. Respective different fluorescence intensities resulting from the differently oriented evanescent fields are correlated with respective different orientations of molecules in the specimen.

Inventors: Hecker; Andreas (Asslar, DE)

Assignee: Leica Microsystems CMS GmbH

International Classification: G01J 3/30 (20060101)

Expiration Date: 2022-06-08 0:00:00