Patent Number: 7,785,795

Title: Method of assaying nucleic acid using labeled nucleotide

Abstract: A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system. This method makes it possible to specifically and accurately assay at least one nucleic acid, which is contained in a single system and can be an unknown nucleic acid and/or a known nucleic acid, with excellent sensitivity, in short time and with ease.

Inventors: Kurata; Shinya (Tokyo, JP), Takatsu; Kyoko (Tokyo, JP), Nakamura; Kazunori (Ibaraki, JP), Kanagawa; Takahiro (Ibaraki, JP)

Assignee: Kankyo Engineering Co., Ltd.

International Classification: C12Q 1/68 (20060101); C12P 19/34 (20060101)

Expiration Date: 8/31/12018