Patent Number: 7,785,833

Title: Methods and means for producing proteins with predetermined post-translational modifications

Abstract: Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules include erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties such that significant amounts of recombinant proteins can be produced that harbor "neural- or brain-type" properties.

Inventors: Opstelten; Dirk Jan Elbertus (Oegstgeest, NL), Kapteyn; Johan Christiaan (Wageningen, NL), Passier; Petrus Christianius Johannes Josephus (Driebergen, NL), Brus; Ronald Hendrik Peter (Voorschoten, NL), Bout; Abraham (Moerkapelle, NL)

Assignee: Crucell Holland B.V.

International Classification: C12N 5/00 (20060101); C07K 14/475 (20060101); C12N 15/00 (20060101); G01N 33/53 (20060101)

Expiration Date: 8/31/12018