Patent Number: 7,786,294

Title: Compositions and methods for amplification and cloning of near full-length viral genome samples

Abstract: A method of producing a polydeoxyribonucleotide molecule by reverse transcriptase polymerase chain reaction wherein the polydeoxyribonucleotide molecule has a length of greater than 5,000 base-pairs is disclosed. The method involves combining two reverse transcriptases followed by two protocols of polymerase chain reaction. This method enable the amplification of large DNAs, such as viruses, from a sample while preserving genetic diversity of the large DNA.

Inventors: Fan; Xiaofeng (St. Louis, MO), Di Bisceglie; Adrian M. (Ladue, MO)

Assignee: Saint Louis University

International Classification: C07H 21/00 (20060101)

Expiration Date: 8/31/12018