Patent Number: 7,787,179

Title: Optical arrangement for the production of a light-sheet

Abstract: The invention is directed to an optical arrangement with a light source for emitting a light bundle and with optical elements for transforming this light bundle into the shape of a light sheet, particularly suitable for illuminating individual planes of a three-dimensional specimen in selective plane illumination microscopy (SPIM). According to the invention, means are provided for varying the cross section of the light sheet, for varying the length of the light sheet and/or for influencing the direction in which individual beam components extending within the light sheet are directed to the specimen substance. This makes it possible to adapt the geometry of the light sheet to the illumination requirements for observing one and the same specimen plane with a plurality of different objectives and, if required, to reduce shadows occurring within the observed specimen plane as a result of the illumination.

Inventors: Lippert; Helmut (Jena, DE), Wald; Matthias (Jena, DE), Radt; Benno (Jena, DE)

Assignee: Carl Ziess MicroImaging GmbH

International Classification: G02B 21/06 (20060101); G02B 9/08 (20060101)

Expiration Date: 8/31/12018