Patent Number: 7,788,039

Title: Quantitation of nucleic acids using growth curves

Abstract: Methods, apparatus, and systems, including computer program products, implement techniques for determining an amount of target nucleic acid ("target") in a sample. Signal data is received for a plurality of cycles of an amplification experiment performed on the target and a standard nucleic acid ("standard"). The signal data includes a series of signal values indicating a quantity of standard present during cycles of the standard amplification, and a series of signal values indicating a quantity of target present during cycles of the target amplification. A target growth curve value is defined using the target signal values and a standard growth curve value is defined using the standard signal values. An initial amount of the target is calculated according to a calibration equation using an initial amount of the standard, and the target and standard growth curve values, where the calibration equation is a nonlinear equation.

Inventors: Vess; Thomas (Raleigh, NC)

Assignee: Roche Molecular Systems, Inc.

International Classification: G06F 19/00 (20060101); C12P 19/34 (20060101); C12Q 1/68 (20060101)

Expiration Date: 8/31/12018