Patent Number: 7,824,928

Title: Hydrophilic chemiluminescent acridinium labeling reagents

Abstract: In accordance with the present invention, it has been discovered that introduction of hydrophilic sulfoalkyl substituents and/or hydrophilic linkers derived from homocysteic acid, cysteic acid, glycine peptides, tetraethylene oxide, and the like, offset the hydrophobicity of the acridinium ring system to produce a more soluble label which can be attached to an antibody at higher loading before precipitation and aggregation problems are encountered. Additional compounds described herein contain linkers derived from short peptides and tetraethylene oxide which increase aqueous solubility due to hydrogen bonding with water molecules. The present invention also embraces reagents for multiple acridinium labeling for signal amplification composed of a peptide bearing several acridinium esters with sulfonate groups at regularly spaced intervals for increased solubility. The invention also embraces assays employing the above-described compounds.

Inventors: Evangelista; Ramon (Laguna Hills, CA), Garrity; Martha (San Clemente, CA)

Assignee: Quest Diagnostics Investments Incorporated

International Classification: G01N 33/533 (20060101); G01N 33/532 (20060101); G01N 21/76 (20060101); C07D 219/02 (20060101); G01N 33/53 (20060101); C07D 219/04 (20060101)

Expiration Date: 2019-11-02 0:00:00