Patent Number: 7,825,218

Title: Solubilization and purification of a target protein fused to a mutant maltose-binding protein

Abstract: Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.

Inventors: Riggs; Paul (Hooksett, NH), Hsieh; Pei-Chung (Topsfield, MA), Walker; Iris (Rowley, MA), Colussi; Paul A. (Gloucester, MA), Ganatra; Mehul (Gloucester, MA), Taron; Christopher H. (Essex, MA)

Assignee: New England Biolabs, Inc.

International Classification: C07K 14/00 (20060101); C07H 21/04 (20060101); C12P 1/00 (20060101); C12P 21/06 (20060101)

Expiration Date: 2019-11-02 0:00:00