Patent Number: 7,825,234

Title: Gene expression modulating element

Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

Inventors: Yi; Hochul (Cary, NC), Klein; Theodore M. (Wilmington, DE), Barton; Joanne E. (Wilmington, DE)

Assignee: Pioneer Hi Bred International Inc

International Classification: C12N 15/63 (20060101); C12N 15/67 (20060101); C12N 15/82 (20060101); A01H 1/00 (20060101)

Expiration Date: 2019-11-02 0:00:00