Patent Number: 7,825,237

Title: Oligonucleotides and analogs labeled with energy transfer dyes

Abstract: Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R.sub.21Z.sub.1C(O)R.sub.22R.sub.28 where R.sub.21 is a C.sub.1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z.sub.1 is either NH, sulfur or oxygen, R.sub.22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R.sub.28 includes a functional group which attaches the linker to the acceptor dye.

Inventors: Lee; Linda (Palo Alto, CA), Spurgeon; Sandra (San Mateo, CA), Rosenblum; Barnett (San Jose, CA)

Assignee: Applied Biosystems, LLC

International Classification: C07H 19/04 (20060101); C07H 21/02 (20060101); C07H 21/04 (20060101); C12Q 1/68 (20060101)

Expiration Date: 2019-11-02 0:00:00