Patent Number: 8,318,438

Title: Aptamer-based assays

Abstract: We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.

Inventors: Vivekananda; Jeevalatha (San Antonio, TX), Kiel; Johnathan L. (Universal City, TX)

Assignee: The United States of America as represented by the Secretary of the Air Force

International Classification: C12Q 1/00 (20060101); G01N 33/569 (20060101); G01N 33/554 (20060101); G01N 33/00 (20060101); C12Q 1/68 (20060101)

Expiration Date: 2021-11-27 0:00:00