Patent Number: 8,492,109

Title: Methods for the delivery of toxins or enzymatically active portions thereof

Abstract: The present invention relates to methods, systems, and kits for intoxicating cells, neuronal and non-neuronal cells, with a toxin or fragment thereof. This is done by subjecting toxin substrate and a lipid or polymeric carrier (e.g., DNA uptake facilitating agent) to one or more cells for use in cell based assays. In an aspect, the methods of the present invention allow for high throughput assays and, as such, for the evaluation of drug candidates.

Inventors: Oyler; George A. (Baltimore, MD), Shoemaker; Charles B. (North Grafton, MA), Kuo; Chuehling (Framingham, MA)

Assignee: Trustees of Tufts College

International Classification: C12Q 1/37 (20060101); C12N 5/07 (20100101); C12Q 1/02 (20060101); C12N 15/00 (20060101)

Expiration Date: 2021-07-23 0:00:00