Patent Number: 8,492,130

Title: FAD-conjugated glucose dehydrogenase gene

Abstract: An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

Inventors: Yada; Takako (Hiroshima, JP), Miyamoto; Koji (Hiroshima, JP), Honda; Michinari (Hiroshima, JP)

Assignee: Ikeda Food Research Co., Ltd.

International Classification: C12Q 1/54 (20060101); C12N 9/00 (20060101); C12N 1/20 (20060101); C12N 15/00 (20060101); C12N 9/96 (20060101)

Expiration Date: 2021-07-23 0:00:00