Patent Number: 8,715,970

Title: Enzymatically catalyzed method of preparing mono-acylated polyols

Abstract: The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.

Inventors: Hauer; Bernhard (Fussgonheim, DE), Kvarnstrom Branneby; Cecilia (Stockholm, SE), Hult; Karl (Stockholm, SE), Magnusson; Anders (Frankfurt, DE), Hamberg; Anders (Bagarmossen, SE)

Assignee: BASF SE

International Classification: C12P 7/62 (20060101); C12N 1/21 (20060101)

Expiration Date: 5/06/12018