Patent Number: 8,715,984

Title: Modified tRNA containing unnatural base and use thereof

Abstract: In the method for introducing a noncanonical amino acid residue into a desired position in a protein, the structure of tRNA is so modified as to have improved affinity for aminoacyl-tRNA synthetase or improved specificity to aminoacyl-tRNA synthetase. An unnatural base is contained at any position in tRNA, whereby the efficiency of aminoacylation of the tRNA with a noncanonical amino acid can be improved.

Inventors: Sekine; Shun-ichi (Tokyo, JP), Fukunaga; Ryuya (Tokyo, JP), Yokoyama; Shigeyuki (Kanagawa, JP), Hirao; Ichiro (Kanagawa, JP), Harada; Yoko (Kanagawa, JP)

Assignee: Riken

International Classification: C12N 9/00 (20060101); C12P 21/06 (20060101)

Expiration Date: 5/06/12018