Patent Number: 8,716,462

Title: Levels and/or sustainability of DNA-based gene expression

Abstract: The invention encompasses methods for improving the level and/or sustainability of expression for a target nucleic acid in a eukaryotic cell comprising: (a) modifying the target nucleic acid to introduce or to comprise signals that limit or constrain the positions of nucleosome cores, and (b) introducing the modified target nucleic acid into the eukaryotic cell, wherein the modified target nucleic acid has improved levels and/or sustainability of expression compared to original unmodified nucleic acid.

Inventors: Fire; Andrew (Washington, DC), Gracey; Lia (Stanford, CA), Lui; Weng-Onn (Stockholm, SE)

Assignee: Carnegie Institution of Washington

International Classification: C07H 21/04 (20060101); C12N 15/00 (20060101); A01N 63/00 (20060101); A61K 48/00 (20060101); A01N 65/00 (20090101)

Expiration Date: 5/06/12018