Patent Number: 8,716,557

Title: Compositions and methods for inhibition of fucosyltransferase and xylosyltransferase expression in plants

Abstract: Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant RNAi construct that provides for the inhibition of expression of .alpha.1,3-fucosyltransferase (FucT) and .beta.1,2-xylosyltransferase (XylT) in a plant. Use of these RNAi constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the G0 glycoform, are also provided.

Inventors: Dickey; Lynn F. (Cary, NC), Cox; Kevin M. (Raleigh, NC), Peele; Charles G. (Apex, NC), Wang; Ming-Bo (Canberra, AU)

Assignee: Synthon Biopharmaceuticals B.V.

International Classification: C12N 15/82 (20060101); A01H 5/00 (20060101); C07H 21/04 (20060101)

Expiration Date: 5/06/12018