Patent Number: 8,722,353

Title: Analytical method for FAB and FAB' molecules

Abstract: A method of measuring acidic species generated by degradation of a Fab or Fab' component of a Fab-PEG or a Fab'-PEG is provided. The method involves: a) cleaving PEG and a linker from the Fab-PEG or Fab'-PEG with an enzyme; b) optionally separating the PEG and linker from the Fab or Fab' to provide isolated Fab or Fab'; and c) quantitatively analyzing acidic species associated with the cleaved Fab or Fab' and/or the cleaved PEG.

Inventors: Smith; Bryan John (Slough, GB), Kirke; Helen Marie (Slough, GB)

Assignee: UCB Pharma, S.A.

International Classification: C12Q 1/37 (20060101)

Expiration Date: 5/13/12018