Patent Number: 8,722,384

Title: Methods and compositions for synthesis of long chain polyunsaturated fatty acids

Abstract: The present invention relates to fatty acid desaturases able to catalyze the conversion of oleic acid to linoleic acid, linoleic acid to .gamma.-linolenic acid, or of alpha-linolenic acid to stearidonic acid. Nucleic acid sequences encoding desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising a desaturase gene, and recombinant host microorganism or animal expressing increased levels of a desaturase are described. Methods for desaturating a fatty acid and for producing a desaturated fatty acid by expressing increased levels of a desaturase are disclosed. Fatty acids, and oils containing them, which have been desaturated by a desaturase produced by recombinant host microorganisms or animals are provided. Pharmaceutical compositions, infant formulas or dietary supplements containing fatty acids which have been desaturated by a desaturase produced by a recombinant host microorganism or animal also are described.

Inventors: Knutzon; Deborah (Granite Bay, CA), Mukerji; Pradip (Gahanna, OH), Huang; Yung-Sheng (Upper Arlington, OH), Thurmond; Jennifer (Columbus, OH), Chaudhary; Sunita (Pearland, TX), Leonard; Amanda Eun-Yeong (Gahanna, OH)

Assignee: Calgene LLC

International Classification: C12N 1/20 (20060101); C07H 21/04 (20060101)

Expiration Date: 5/13/12018