Patent Number: 8,795,986

Title: Microbial method for the biotransformation of colchicinoid compounds

Abstract: The invention relates to a method for the Bio-transformation of Thiocolchicine a colchicinoid compound into its glycosylated form using a selective microorganism Providencia vermicola. Use of bacterial strain, Providencia vermicola for microbial bio-transformation of thiocolchicine (TCN) to their corresponding 3-0-glycosyl derivative as well as specific strains of that species Providencia vermicola for microbial transformation of other colchicinoid compounds such as colchicine, thiocolchicine, 3-demethylcolchicine, 3-demethylthiocolchicine and N-deacetylthiocolchicine to its corresponding glycosylated form and subsequent isolation of the transformed compounds from the bacterial culture medium is disclosed. Providencia vermicola (MTCC 5578) a gram negative bacteria has been identified having trans-glycosylation ability and convert thiocolchicine (TCN) to Thiocolchicocide (TCS).

Inventors: Prabakaran; Krishnamurthy (Karnataka, IN), Kanagavelrajan; Sankarasubramanian (Karnataka, IN), Kannamapuram; Nallasamy Mohanasundaram (Karnataka, IN), Kooakarakkaran; Ayyappan Hariharan (Karnataka, IN), Poliseti; Bhimaraju (Karnataka, IN)

Assignee: Sequent Scientific Ltd.

International Classification: C12P 19/56 (20060101); C12N 1/20 (20060101)

Expiration Date: 8/05/12018