Patent Number: 8,815,509

Title: Fluorescence-based assay for the rapid detection and quantification of deoxyribonucleoside triphosphates

Abstract: The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3' exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation.

Inventors: Wilson; Peter M. (Pasadena, CA), Ladner; Robert D. (Santa Monica, CA)

Assignee: University of Southern California

International Classification: C12Q 1/68 (20060101); C07H 19/04 (20060101); C07H 21/02 (20060101); G01N 33/52 (20060101); G01N 21/66 (20060101); G01N 21/64 (20060101); C12P 19/34 (20060101); C07H 21/04 (20060101); G01N 31/22 (20060101)

Expiration Date: 8/26/12018