Patent Number: 8,815,590

Title: Composition and method for differentiation of human embryonic stem cells

Abstract: Described are methods for inducing differentiation of a human embryonic stem cell or a population of human embryonic stem cells toward a cell or population of cells characteristic of the definitive endoderm, the method comprising incubating the cell or population of cells with a GSK-3 inhibitor. Also described are methods for inducing differentiation of a cell or population of cells, characteristic of the definitive endoderm, towards a hepatocyte-like cell or a population of hepatocyte-like cells, and methods for inducing differentiation of a human embryonic stem cell or a population of human embryonic stem cells toward a hepatocyte-like cell or a population of hepatocyte-like cells. Further described are cells obtained by the methods and uses thereof in therapy and toxicity screening.

Inventors: Bone; Heather K. (Winterbourne, GB), Tosh; David (Bath, GB), Welham; Melanie J. (Whitley, GB)

Assignee: Stem Cells for Safer Medicines Limited

International Classification: C12N 5/00 (20060101)

Expiration Date: 8/26/12018