Patent Number: 8,822,217

Title: Method for cultivating tendon cells from pluripotent cells of mesenchymal origin

Abstract: A method for cultivating tendon cells from non-embryonic pluripotent cells of mesenchymal origin includes the cultivation of isolated cells in a culture medium under standard culture conditions in a culture vessel. In order to increase the collagen secretion, before their complete confluence, the cells are further cultivated in a culture medium mixed with ascorbic acid and/or ascorbic acid-2-phosphate in a concentration of 25 to 75 .mu.g/ml and are subjected to hyperosmolar treatment in a culture medium whose osmolarity is adjusted to 350 to 500 mosmol/l.

Inventors: Bauer; Hans-Christian (Osterreich, AT), Tempfer; Herbert (Osterreich, AT)

Assignee: Angewandte Biotechnologie GmbH

International Classification: C12N 5/077 (20100101)

Expiration Date: 9/02/12018